Insulin inhibits changes in the phospholipid profiles in sciatic nerves from streptozocin-induced diabetic rats: A phosphorus-31 magnetic resonance study |
| |
| Institution: | 1. Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Room 3128, Building 37, Bethesda, MD 20892, United States;2. Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Room 5120, Building 37, Bethesda, MD 20892, United States;1. Diabetes and Cardiovascular Research Center, Department of Medicine, University of Missouri Columbia, School of Medicine, Columbia, MO 65212, USA;2. Division of Nephrology and Hypertension, Department of Medicine, University of Missouri Columbia, School of Medicine, Columbia, MO 65212, USA;3. Department of Medical Pharmacology and Physiology, University of Missouri Columbia, School of Medicine, Columbia, MO 65212, USA;4. Research Service Harry S Truman Memorial Veterans Hospital, University of Missouri School of Medicine, Columbia, MO 65212, USA;5. Dalton Cardiovascular Research Center, University of Missouri School of Medicine, Columbia, MO 65212, USA;1. Department of Ophthalmology, King George’s Medical University, Lucknow, India;2. Developmental Toxicology Division, CSIR- Indian Institute of Toxicology Research, Lucknow, India;3. Department of Ophthalmology, Pallas Klinik, Olten, Switzerland;4. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic;5. 2nd Department of Internal Medicine, St. Anne´s University Hospital and Masaryk University, Brno, Czech Republic |
| |
| Abstract: | Sciatic nerve phospholipids obtained from insulin-treated streptozocin-induced diabetic, non-treated streptozocin-induced diabetic, and healthy, control male Sprague-Dawley rats after eighteen weeks of diabetes were studied by 31P NMR spectrometry. Eleven phospholipids resonances were identified as follows: Phosphatidic acid (Chemical shift, 0.30 ppm), dihydrosphingomyelin (0.13 ppm), ethanolamine plasmalogen (0.07 ppm), phosphatidylethanolamine (0.03 ppm), phosphatidylserine (?0.05 ppm), sphingomyelin (?0.09 ppm), lysophosphatidylcholine (?0.28 ppm), phosphatidylinositol (?0.30 ppm), alkylacylglycerophosphorylcholine (?0.78 ppm), choline plasmalogen (?0.80 ppm), and phosphatidylcholine (?0.84 ppm). Diabetic rats showed that phosphatidylcholine was significantly elevated p > 0.05, and ethanolamine plasmalogen and choline plasmalogen were significantly lower when compared with both control and insulin treated rats. The choline ratio (choline-containing phospholipids over noncholine phospholipids) was significantly elevated in the diabetic group, when compared with both control and insulin-treated groups. The ethanolamine ratio (ethanolamine-containing phospholipids over nonethanolamine phospholipids) and the ratio of the ethanolamine ratio over the choline ratio, was significantly elevated in the control and the insulin-treated groups when compared with the diabetic group. The presence of phosphatidic acid and the significance in phosphatidylcholine and ethanolamine plasmalogen, suggested that insulin had a role in the phosphatidylcholine metabolism in the rat nerve. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
