The effect of angiotensin 1-7 on tyrosine kinases activity in rat anterior pituitary |
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Authors: | Rebas Elzbieta Zabczyńska Joanna Lachowicz Agnieszka |
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Institution: | Department of Molecular Neurochemistry, Medical University of Lodz, Poland. elkar@zdn.am.lodz.pl |
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Abstract: | Angiotensin 1-7 (Ang 1-7) is a peptide originated from Ang II. It is known that in vessels Ang 1-7 shows opposite effects to Ang II. Ang 1-7 can modify processes of proliferation. However, Ang 1-7 action in pituitary gland cells was never studied. Moreover, the specific binding sites for Ang 1-7 are still unknown. The aim of this study was to examine the effects of Ang 1-7 on tyrosine kinases (PTKs) activity in the anterior pituitary. The reaction of phosphorylation was carrying out in presence of different concentration of Ang 1-7 and losartan (antagonist of AT1 receptor) and PD123319 (antagonist of AT2). Our results show that Ang 1-7 inhibited activity of PTK to 60% of basic activity. Losartan did not change the Ang 1-7-induced changes in PTKs activity. The presence of PD123319 together with Ang 1-7 caused stronger inhibition PTKs activity than Ang 1-7 alone. These observations suggest that Ang 1-7 binds to the novel, unknown, specific for this peptide receptor. |
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Keywords: | Angiotensin 1-7 Tyrosine kinases Losartan PD123319 Angiotensin receptors |
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