Type analysis of oligosaccharide chains on human and murine MHC class II alpha chains by the lectin-nitrocellulose sheet method |
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Authors: | Y U Katagiri S Kijimoto-Ochiai T Hatae H Okuyama |
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Affiliation: | Institute for Immunological Science, Hokkaido University, Sapporo, Japan. |
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Abstract: | 1. The microheterogeneous alpha molecules of class II antigen, DR molecules obtained from human B cell line and I-A molecules from mouse B cell hybridoma cell line, were separated by 2-D PAGE, transferred onto NC sheets and N-linked oligosaccharide types were analyzed by staining with P.O./lectins. 2. This is the first report to show directly the type of oligosaccharide chain corresponding to each spot separated by 2-D PAGE. The glycosylation patterns of class II alpha chains in human and mouse were compared. |
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