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Rapid detection of glycyrrhizin by immunochromatographic assay
Authors:Putalun Waraporn  Tanaka Hiroyuki  Shoyama Yukihiro
Affiliation:Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand.
Abstract:An immunochromatographic assay was developed for detecting glycyrrhizin (1). The qualitative assay is based on a competitive immunoassay using anti-1 monoclonal antibody (MAb) and a detector reagent that contains colloidal gold particles coated with anti-1 MAb. The immunochromatographic strip test, which has a detection limit of 250 ng/mL, is useful as a rapid screening method for detecting glycyrrhizin in plants, biological fluids and food samples.
Keywords:immunochromatographic strip test  monoclonal antibody  glycyrrhizin
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