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Developments in cell culture systems for human pluripotent stem cells
Authors:Weiwei Liu  Chunhao Deng  Carlos Godoy-Parejo  Yumeng Zhang  Guokai Chen
Abstract:Human pluripotent stem cells (hPSCs) are important resources for cell-based therapies and pharmaceutical applications. In order to realize the potential of hPSCs, it is critical to develop suitable technologies required for specific applications. Most hPSC technologies depend on cell culture, and are critically influenced by culture medium composition, extracellular matrices, handling methods, and culture platforms. This review summarizes the major technological advances in hPSC culture, and highlights the opportunities and challenges in future therapeutic applications.
Keywords:Human pluripotent stem cells  Human embryonic stem cells  Cell culture  Culture medium  Stem cell niche  Signal transduction  Embryoid bodies
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