Purification and characterisation of the breast cancer metastasis suppressor, BRMS1 |
| |
Authors: | Joanne Smith Riffat Naseem Michelle Webb |
| |
Affiliation: | aUniversity of Manchester, Faculty of Medical and Human Sciences, Department of Medical Genetics, Stopford Building, Manchester M13 9PT, UK;bCardiff University, Department of Medical Biochemistry and Immunology, School of Medicine, Tenovus Building, Heath Park, Cardiff CF14 4XN, UK |
| |
Abstract: | The breast cancer metastasis suppressor 1 (BRMS1) is a member of a family of proteins that actively suppress tumour metastasis. Understanding BRMS1 mediated metastasis suppression is critical to the development of new therapies designed to prevent and treat patients with late stage breast cancer. To aid research into the functional aspects that underpin BRMS1 mediated metastasis suppression we have expressed and purified recombinant BRMS1 and produced BRMS1 polyclonal antibodies. Using these antibodies to immunoprecipitate endogenous BRMS1 containing complexes from MCF7 breast cancer cell lines we have identified, by mass spectrometry, the small heat shock protein Hsp27 in complex with BRMS1. We also show that the expression of both BRMS1 and Hsp27 are inversely correlated with metastatic potential. |
| |
Keywords: | Antibodies BRMS1 Metastasis Hsp27 |
本文献已被 ScienceDirect 等数据库收录! |
|