Femtosecond spectroscopic observations of initial intermediates in the photocycle of the photoactive yellow protein from Ectothiorhodospira halophila. |
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| Authors: | S Devanathan A Pacheco L Ujj M Cusanovich G Tollin S Lin N Woodbury |
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| Affiliation: | Department of Biochemistry, University of Arizona, Tucson, Arizona 85721, USA. |
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| Abstract: | Femtosecond time-resolved absorbance measurements were used to probe the subpicosecond primary events of the photoactive yellow protein (PYP), a 14-kD soluble photoreceptor from Ectothiorhodospira halophila. Previous picosecond absorption studies from our laboratory have revealed the presence of two new early photochemical intermediates in the PYP photocycle, I(0), which appears in
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