The regeneration of coenzymes using immobilized hydrogenase |
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| Authors: | Alexander M Klibanov Austin V Puglisi |
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| Institution: | (1) Laboratory of Applied Biochemistry Department of Nutrition and Food Science, Massachusetts Institute of Technology, 02139 Cambridge, MA, USA |
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| Abstract: | Summary A new enzymatic approach to the regeneration of coenzymes has been developed. It uses the reduction of natural or artificial cofactors with H2, catalyzed by hydrogenase activity in immobilized whole cells ofA. eutrophus. The method has been employed for regeneration of such coenzymes as NAD+, FMN, phenazine methosulfate, Janus green, methylene blue, and 2,6-dichlorophenol-indophenol. |
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