Genomic identification of microbial species adhering to maxillofacial prostheses and susceptibility to different hygiene protocols |
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| Authors: | Juliana Barchelli Pinheiro Marina Peris Vomero Cássio do Nascimento Evandro Watanabe Helena de Freitas Oliveira Paranhos Neide Pena Coto |
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| Affiliation: | 1. Department of Dental Materials and Prostheses, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil;2. Department of Restorative Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil;3. Department of Maxillofacial Surgery, Prostheses and Traumatology, School of Dentistry of Sao Paulo, University of Sao Paulo, Sao Paulo, Brazil |
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| Abstract: | This study investigated the microbial colonization of maxillofacial prostheses and support tissues using the Checkerboard DNA–DNA hybridization method, and the efficacy of 0.12% chlorhexidine gluconate, 10% Ricinus communis solutions, or brushing, on colony forming unit (CFU) reduction in monospecies biofilms (Candida glabrata, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Enterococcus faecalis, and Pseudomonas aeruginosa) formed on two silicones (MDX 4-4210 and Bio-Skin). Biofilm was harvested from 43 maxillofacial prosthesis wearers for detection of 38 species of microorganisms. The CFU counts of the six above mentioned species were recorded after using the hygiene protocols. All 38 investigated species were identified in prostheses and tissues, with a higher prevalence in the prostheses. 0.12% chlorhexidine gluconate immersion showed the greatest antimicrobial effectiveness, followed by mechanical brushing protocols. MDX 4-4210 silicone produced lower CFU counts than Bio-Skin. |
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| Keywords: | Maxillofacial prosthesis silicones prosthesis cleansers microbial biofilm |
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