Inhibition of [3H]Spiroperidol Binding by In Vitro Addition of Ethanol |
| |
Authors: | Robert E Hruska Ellen K Silbergeld |
| |
Institution: | Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, Nottingham NG7 2UH, U.K. |
| |
Abstract: | : The in vitro addition of ethanol at concentrations greater than 0.2% produced an inhibition of 3H]spiroperidol binding. This inhibition was competitive, lowering the affinity without altering the maximum number of sites. Both the rates of association and dissociation were altered such that the ligand spent less time attached to the receptor. Since the binding was competitive, little inhibition was seen at ligand concentrations greater than 300 pm . These results are important for in vitro studies when drugs are dissolved in ethanol, and also for in vivo studies on the effects of ethanol. |
| |
Keywords: | Key words: DA receptors—Ethanol—[3H]Spiroperidol binding |
本文献已被 PubMed 等数据库收录! |
|