Production of succinic acid from sucrose and sugarcane molasses by metabolically engineered Escherichia coli |
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Authors: | Chan Sitha Kanchanatawee Sunthorn Jantama Kaemwich |
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Institution: | School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Ave., Suranaree, Muang, Nakhon Ratchasima 30000, Thailand |
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Abstract: | Sucrose-utilizing genes (cscKB and cscA) from Escherichia coli KO11 were cloned and expressed in a metabolically engineered E. coli KJ122 to enhance succinate production from sucrose. KJ122 harboring a recombinant plasmid, pKJSUC, was screened for the efficient sucrose utilization by growth-based selection and adaptation. KJ122-pKJSUC-24T efficiently utilized sucrose in a low-cost medium to produce high succinate concentration with less accumulation of by-products. Succinate concentrations of 51 g/L (productivity equal to 1.05 g/L/h) were produced from sucrose in anaerobic bottles, and concentrations of 47 g/L were produced in 10 L bioreactor within 48 h. Antibiotics had no effect on the succinate production by KJ122-pKJSUC-24T. In addition, succinate concentrations of 62 g/L were produced from sugarcane molasses in anaerobic bottles, and concentrations of 56 g/L in 10 L bioreactor within 72 h. These results demonstrated that KJ122-pKJSUC-24T would be a potential strain for bio-based succinate production from sucrose and sugarcane molasses. |
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Keywords: | Succinate Escherichia coli Metabolic engineering Sucrose Molasses |
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