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Monoclonal antibodies against pertussis toxin subunits

Authors:	Massimo Bigio Roberta Rossi Daniele Nucci Maria Giuseppina Borri Guido Antoni Antonella Bartoloni Rino Rappuoli

Affiliation:	Center of Marine Biotechnology, University of Maryland, Baltimore, MD 21202, U.S.A.;Department of Microbiology, University of Maryland, College Park, MD 20742, U.S.A.;Department of Bacteriology and Serology, Research Institute for Microbial Diseases, Osaka University, Yamada-oka, Suita, Osaka 565, Japan

Abstract:	Abstract Twenty monoclonal antibodies (mAbs) reacting with cholera toxin (CT) of Vibrio cholerae strain 569B were characterized in cross-section and G_M1 ganglioside inhibition assays. MAbs were characterized by reaction with CT and Escherichia coli heat-labile porcine strain (LT_p) and human strain (LTh) enterotoxins, and by G_M1 ganglioside inhibition of mAb binding. Eight of 10 CT-A specific and 3 of 10 CT-B-specific mAbs cross-reacted with LTh and LTp. G_M1 ganglioside inhibited reactions of the CT-B cross-reacting antibodies. Results showed that these epitodes common to the B subunit of CT and LT are located in or near the G_M1 ganglioside binding region, and that the G_M1 ganglioside-binding region of LT differs from that of CT.

Keywords:	Vibrio cholerae Cholera toxin Escherichia coli LT
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