Effect of a nuclepolyhedrovirus of Autographa californica expressing the enhancin gene of Trichoplusia ni granulovirus on T. ni larvae |
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Authors: | M Cristina Del Rinc n-Castro Jorge E Ibarra |
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Institution: | M. Cristina Del Rincó,n-Castro ,Jorge E. Ibarra |
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Abstract: | A recombinant Autographa californica nucleopolyhedrovirus (AcMNPV) strain showing higher virulence against Trichoplusia ni larvae than the wild-type virus was developed. The 'enhancin' (VEF) gene of T. ni granulovirus (TnGV) and the AcMNPV polyhedrin gene were cloned into the baculovirus transfer vector pAcUW31. This plasmid and AcMNPV BacPAK6 DNA were co-transfected into the BTI-Tn5B1-4 cell line. A recombinant AcMNPV strain (BacVEFPol) was purified, amplified, and bioassayed against T. ni first instar larvae. Its estimated LC50 (0.184?OB/mm2) was 2.18 times lower than the LC50 estimated for the wild-type AcMNPV (0.402?OB/mm2). Likewise, an LT50 of 67.7?h was estimated for the recombinant AcMNPV strain while the LT50 of wild-type AcMNPV was estimated at 81.9?h. This indicates a 17.4% reduction of the time required to kill the larvae. The higher virulence of the recombinant strain, evidenced by its LC50 and LT50 values being lower than those of the wild-type strain, indicates that the VEF protein is expressed properly and may be occluded in the OBs. |
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Keywords: | Enhancin polyhedrin VEF AcMNPV TnGV Trichoplusia ni |
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