Through-bond correlation of adenine H2 and H8 protons in unlabeled DNA fragments by HMBC spectroscopy |
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| Authors: | Maria J. P. van Dongen Sybren S. Wijmenga Ramón Eritja Fernando Azorín Cornelis W. Hilbers |
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| Affiliation: | (1) NSR Centre for Molecular Structure, Design, and Synthesis, Laboratory of Biophysical Chemistry, University of Nijmegen, Toernooiveld, 6525 ED Nijmegen, The Netherlands;(2) Department of Molecular and Cellular Biology, Centro de Investigación y Desarrollo-CSIC, Jorge Girona Salgado 18-26, E-08034 Barcelona, Spain;(3) Present address: Department of Medical Biochemistry and Biophysics, University of Umeå, S-90187 Umeå, Sweden;(4) Present address: European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69012 Heidelberg, Germany |
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| Abstract: | Summary A new application of the HMBC experiment is presented that provides a useful means to discriminate between H2 and H8 proton resonances, to assign the base proton resonances to the various residue types and, most importantly, to correlate the H2 and H8 protons for adenine or inosine residues in natural abundance 13C fragments. The utility of this experiment is demonstrated for an unlabeled DNA 20-mer. Thanks to the obtained results, preliminary conclusions could be drawn regarding the molecular conformations of the non-canonical G/I-A base pairs in the hairpin formed by this fragment. |
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| Keywords: | Through-bond H2-H8 correlation Unlabeled DNA HMBC Two-dimensional NMR G-A base pairs |
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