Antibody-based surface plasmon resonance detection of intact viral pathogen |
| |
Authors: | Baac Hyoungwon Hajós József P Lee Jennifer Kim Donghyun Kim Sung June Shuler Michael L |
| |
Affiliation: | Department of Biomedical Engineering, Cornell University, Ithaca, New York 14853, New York, USA. |
| |
Abstract: | Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two-channel (reference & test) micro-fluidic SPR system is used for reliable measurement. Bio-specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors. |
| |
Keywords: | intact pathogen surface plasmon resonance: baculovirus tobacco mosaic virus |
本文献已被 PubMed 等数据库收录! |
|