Isolation and growth of the phototrophic bacterium Rhodopseudomonas palustris strain B1 in sago-starch-processing wastewater |
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Authors: | Getha K Vikineswary S Chong VC |
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Institution: | (1) Institute of Postgraduate Studies and Research, University of Malaya, 50603 Kuala Lumpur, Malaysia |
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Abstract: | An indigenous strain of the purple non-sulphur phototrophic bacterium, Rhodopseudomonas palustris strain B1, was selected for the utilization and treatment of wastewater from a sago-starch-processing decanter. Growth of Strain B1 under anaerobic–light conditions in the carbohydrate-rich effluent was optimized by using 50% (v/v) effluent diluted in a basal minimal mineral medium with the addition to 0.1% (w/v) yeast extract. The optimum level of nitrogen source supplement, ammonium sulphate, was 1.0g/l. Highest cell mass concentration was achieved by using tungsten lamps as the light source with a light intensity of 4 klux. Under these optimal conditions, a maximum biomass of about 2.5g dry cell/l with a pigment content of about 1.1mg carotenoid/g dry weight cell was achieved after 96h of anaerobic cultivation. There was a 77% reduc n the chemical oxygen demand (COD) of the effluent. A cell yield of about 0.59g dry weight cell/g COD was obtained. |
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Keywords: | Phototrophic bacteria purple nonsulphur bacteria Rhodopseudomonas palustris single cell protein wastewater bioremediation |
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