Accuracy and quality of massively parallel DNA pyrosequencing |
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Authors: | Susan M Huse Julie A Huber Hilary G Morrison Mitchell L Sogin David Mark Welch |
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Institution: | (1) Josephine Bay Paul Center, Marine Biological Laboratory at Woods Hole, MBL Street, Woods Hole, MA 02543, USA |
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Abstract: | Background Massively parallel pyrosequencing systems have increased the efficiency of DNA sequencing, although the published per-base
accuracy of a Roche GS20 is only 96%. In genome projects, highly redundant consensus assemblies can compensate for sequencing
errors. In contrast, studies of microbial diversity that catalogue differences between PCR amplicons of ribosomal RNA genes
(rDNA) or other conserved gene families cannot take advantage of consensus assemblies to detect and minimize incorrect base
calls. |
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Keywords: | |
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