Phosphate compounds as iron chelators in animal cell cultures |
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Authors: | Leif Rasmussen Hans Toftlund |
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Institution: | (1) Institute of Anatomy and Cytology, Odense University, 5230 Odense M, Denmark;(2) Institute of Chemistry, Odense University, 5230 Odense M, Denmark |
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Abstract: | Summary We have studied the capacity of a number of phosphate compounds to act in the double role as a phosphate source and a detoxifier
of ferric chloride hydroxo compounds, i.e. as Fe(III) chelators. The tested compounds were: orthophosphate, trimetaphosphate,
α-glycerophosphate, β-glycerophosphate, phytic acid, and phosphorylcholine; the test organism the ciliate protozoonTetrahymena thermophila, an animal cell; and the nutrient medium was synthetic, consisting solely of low-molecular-weight compounds.
We assessed growth rates of cells in two experimental series. First, phosphate-starved cells were exposed to the tested phosphate
compound as the only phosphate source and the ferric chloride concentrations were varied stepwise from 0 to 1000μM. Second, we offered the cells orthophosphate as a phosphate source and selected phosphate compounds as chelators.
The cell growth results allow the following conclusions: orthophosphate, trimetaphosphate, α-glycerophosphate, and β-glycerophosphate
are excellent phosphate sources; trimetaphosphate, α-glycerophosphate, β-glycerophosphate, and phytic acid are excellent Fe(III)
chelators; of the tested compounds trimetaphosphate, α-glycerophosphate, and β-glycerophosphate are excellent in the double
role as a phosphate source and a ferric chloride hydroxo detoxifier, i.e. as a Fe(III) chelator. |
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Keywords: | extracellular phosphate compounds Fe(III) detoxification Fe(III) utilization |
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