Regulation of protein synthesis by estradiol 17β, dexamethasone and insulin in primary cultured Xenopus hepatocytes

Changes in the protein synthesis of Xenopus hepatocytes caused by insulin, estradiol-17β (estradiol) and dexamethasone were studied by using a primary culture in serum-free medium. All of these hormones stimulated the synthesis of secretory and intracellular proteins. Dexamethasone induced or stimulated the synthesis of many proteins (though limited in number), whereas estradiol induced or stimulated relatively few proteins, including the yolk precursor protein vitellogenin. The majority of these proteins differed in molecular weight and/or isoelectric point. When hepatocytes were treated with both steroids, most of the proteins were synthesized at the rates expected from the single treatment of the respective steroids. Thus, each steroid selectively stimulated the synthesis of its specific proteins. However, exceptional proteins were observed, whose syntheses were stimulated only by double treatment. In contrast, insulin seemed to cause an overall increase in individual secretory protein synthesis.