Residues within the HFRIGC sequence of HIV-1 vpr involved in growth arrest activities. |
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Authors: | J M Berglez L A Castelli S A Sankovich S C Smith C C Curtain I G Macreadie |
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Institution: | Biomolecular Research Institute, Parkville, Victoria, Australia. |
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Abstract: | HIV-1 Vpr is a virion-associated protein that can cause growth arrest when produced inside the cell but when added externally it can cause cell death. Employing the yeast model system, the C-terminal domain, in particular the sequence HFRIGCRHSRIG (Vpr(71-82)), is essential for both the growth arrest and cytocidal activities. Conservation of this sequence in HIV-2 and SIV suggests that these residues may be functionally important. Using site-directed mutagenesis we show that the most highly conserved aa residues, His71 and Gly75, were important for the cell cycle inhibitory effects. In contrast, we show that the wild-type Vpr(71-82) peptide and three variants of this peptide with Gly75 changed to Ser, Ala, and Ile all exhibited the same cytocidal activity suggesting that the intracellular and extracellular effects are unrelated. |
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