The biochemistry of blister fluid from pediatric burn injuries: proteomics and metabolomics aspects |
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| Authors: | Tuo Zang Daniel A Broszczak James A Broadbent Leila Cuttle Haitao Lu |
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| Affiliation: | 1. Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Kelvin Grove, Australia;2. School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia;3. Wound Management Innovation Co-operative Research Centre, West End, Australia;4. Centre for Children’s Burns and Trauma Research, Queensland University of Technology, Institute of Health and Biomedical Innovation at the Centre for Children’s Health Research, South Brisbane, Australia |
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| Abstract: | Burn injury is a prevalent and traumatic event for pediatric patients. At present, the diagnosis of burn injury severity is subjective and lacks a clinically relevant quantitative measure. This is due in part to a lack of knowledge surrounding the biochemistry of burn injuries and that of blister fluid. A more complete understanding of the blister fluid biochemistry may open new avenues for diagnostic and prognostic development. Burn insult induces a highly complex network of signaling processes and numerous changes within various biochemical systems, which can ultimately be examined using proteome and metabolome measurements. This review reports on the current understanding of burn wound biochemistry and outlines a technical approach for ‘omics’ profiling of blister fluid from burn wounds of differing severity. |
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| Keywords: | pediatric burns proteomics metabolomics mass spectrometry nuclear magnetic resonance blister fluid |
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