Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport |
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| Authors: | Peter J I van de Vondervoort Marco J L de Groot George J G Ruijter Jaap Visser |
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| Institution: | (1) Section Molecular Genetics of Industrial Microorganisms, Wageningen University, Dreijenlaan 2, 6703 HA Wageningen, The Netherlands;(2) Present address: Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands;(3) Present address: Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands;(4) Present address: Department Clinical Genetics, Eramus Medical Centre, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands;(5) Present address: FGT Consultancy, P.O. Box 396, 6700 AJ Wageningen, The Netherlands |
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| Abstract: | Aspergillus niger is known for its biotechnological applications, such as the use of xylanase enzyme for the degradation of hemicellulose. Depending on culture conditions, several polyols may also be accumulated, such as xylitol during D-xylose oxidation. Also during industrial fermentation of xylose for the production of fuel ethanol by recombinant yeast, xylitol is a by-product. We studied xylitol metabolism by isolating mutants that have impaired xylitol-mediated repression. Genetic and biochemical characterisation revealed that one of these mutants was affected not only in xylitol-mediated carbon repression, but also had impaired xylitol transport.The first two authors have contributed equally to this work. |
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| Keywords: | Mutant selection Xylitol transport Gene regulation Pentose repression Xylose fermentation |
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