Activity of AMP-Regulated Protein Kinase and AMP-Deaminase in the Heart of Mice Fed High-Fat Diet |
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Authors: | Iwona Rybakowska Ewa M. Slominska Paweł Romaszko Marcin Lipiński Paulina Żukowska Ryszard T. Smolenski |
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Affiliation: | 1. Department of Biochemistry and Clinical Physiology, Medical University of Gdansk, Gdansk, Polandiwonar@gumed.edu.pl;3. Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland;4. Department of Pharmaceutical Biochemistry, Medical University of Gdansk, Gdansk, Poland |
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Abstract: | AMP-regulated protein kinase (AMPK) is involved in numerous regulatory processes and its role in control of cardiac energy metabolism is particularly important. This activity could be affected by AMP-deaminase (AMPD) since substrate of AMPD is AMPK activator. Hearts of male mouse, fed for six weeks with normal or high-fat diet, were fractionated to enrich AMPK activity. Purified fraction was incubated with AMARA peptide for up to 5 minutes and then conversion of AMARA to pAMARA was determined by liquid chromatography—mass spectrometry (LC/MS) using mass detector. Activity of AMPK in heart was 0.038 ± 0.012 pmol/min/mg protein for mice fed high-fat diet and that was not different to control (0.032 ± 0.01 pmol/min/mg protein). We observed change in AMPD activity. It was 5.39 ± 1.5 nmol/mg tissue/min in heart of mice fed high-fat diet while in heart of mice fed low-fat diet it was 2.29 ± 0.32 nmol/mg tissue/min. Data we present indicate that while total AMPK activity is not changed decrease in AMPD activity may affect AMPK signaling in diabetic heart. |
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Keywords: | AMP-regulated protein kinase AMP-deaminase high-fat diet diabetes |
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