Evaluation of antigenotoxicity effects of umbelliprenin on human peripheral lymphocytes exposed to oxidative stress |
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Authors: | Fatemeh Soltani Fatemeh Mosaffa Mehrdad Iranshahi Gholamreza Karimi Mohammad Malekaneh Fatemeh Haghighi Javad Behravan |
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Affiliation: | (1) Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran;(2) Department of Pharmacognosy and Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, P. O. Box 91775-1365, Mashhad, Iran;(3) Department of Pharmacodinamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;(4) Medical Toxcicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran;(5) Department of Clinical Biochemistry, Birjand University of Medical Sciences, Birjand, Iran;(6) Department of Pathology, Birjand University of Medical Sciences, Birjand, Iran; |
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Abstract: | The protective properties of a prenylated coumarin, umbelliprenin (UMB), on the human lymphocytes DNA lesions were tested. Lymphocytes were isolated from blood samples taken from healthy volunteers. DNA breaks and resistance to H2O2-induced damage were measured using a single-cell microgel electrophoresis technique under alkaline conditions (comet assay). Human lymphocytes were incubated in UMB (10, 25, 50, 100, 200, and 400 μM) alone or a combination of different concentrations of UMB (10, 25, 50, 100, 200, and 400 μM) and 25 μM H2O2. Untreated cells, ascorbic acid (AA; 25, 50, 100, 200, and 400 μM) and H2O2 (25 μM) were considered as negative control, positive control, and the standard antioxidant agent for our study, respectively. Single cells were analyzed with “TriTek Cometscore version 1.5” software. The DNA damage was expressed as percent tail DNA. UMB exhibited a concentration-dependent increase in protection activity against DNA damage induced by 25 μM H2O2 (from 67.28% to 39.17%). The antigenotoxic activity of AA, in the range 0–50 μM, was greater than that of UMB. However, no significant difference (p > 0.05) in the protective activity was found between UMB and AA at concentrations of approximately higher than 50 μM. |
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Keywords: | Antigenotoxicity Comet assay Umbelliprenin |
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