Regulation of fructosyltransferase activity by carbohydrates, in solution and immobilized on hydroxyapatite surfaces |
| |
Authors: | Steinberg Doron Rozen Ramona Bromshteym Moshe Zaks Batia Gedalia Itzhak Bachrach Gilad |
| |
Institution: | Department of Oral Biology, Faculty of Dentistry, Hebrew University-Hadassah, PO Box 12272, Jerusalem 91120, Israel. dorons@cc.huji.ac.il |
| |
Abstract: | We tested the effect of several carbohydrates on the activity of cell-free fructosyltransferases (FTF) in solution and immobilized onto hydroxyapatite (HA) and found an inhibitory dose-dependent effect of glucose on FTF activity, both on the surface and in solution. Glucose at 160 mM inhibits FTF activity by 75% both on HA and in solution. Fructose at 160 mM inhibited FTF activity by 25% in solution and by 15% on HA. Levan inhibited FTF activity by 30% in solution, while dextrans and inulin had a limited effect on FTF activity. Circular dichroism and infrared analysis demonstrated no major changes in the chemical structure of fructans synthesized by cell-free FTF on HA and in solution, in the presence or absence of glucose. However, as verified by size-exclusion chromatography, glucose inhibited the synthesis of high molecular-weight fructans. The results indicate that glucose, a byproduct of the FTF enzymatic reaction, is the main carbohydrate affecting FTF activity. Selective inhibition of high molecular-weight fructan production by glucose, may indicate that two mechanisms are involved in the synthesis of fructans, both in solution and on the surface. |
| |
Keywords: | Fructosyltransferase Fructans Biofilm Hydroxyapatite Dental plaque |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|