Trifluralin herbicide-induced resistance of melon to fusarium wilt involves expression of stress- and defence-related genes |
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| Authors: | MAYA LOTAN-POMPAN RON COHEN ODED YARDEN VITALY PORTNOY YOSEF BURGER NURIT KATZIR |
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| Affiliation: | Department of Vegetable Crops, Newe Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat-Yishay 30095, Israel;
Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, PO Box 12, Rehovot 71600, The Hebrew University of Jerusalem, Israel |
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| Abstract: | To identify genes involved in trifluralin herbicide-induced resistance of melon to Fusarium oxysporum f. sp. melonis , suppression subtractive hybridization (SSH) and cDNA-amplified fragment-length polymorphism (cDNA-AFLP) were used. A total of 123 clones—60 of which have never been isolated from melon—were isolated, sequenced and annotated. A significant proportion (35%) of the total 123 clones exhibited similarity to genes that have been formerly described as stress- or defence-related. Thirty-two selected clones were subjected to a detailed expression analysis, one-third of which were found to be up-regulated in response to trifluralin treatment and/or fusarium inoculation. The putative roles of seven of these clones in stress are discussed. Furthermore, the expression of four stress-related and up-regulated genes was enhanced when the plants were subjected to salinity stress, suggesting that trifluralin induces a general stress response which protects the plant against fusarium wilt. |
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