Electron microscope analysis of mouse and rabbit globin and immunoglobulin gene sequences |
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| Authors: | J-D Rochaix F Rougeon B Mach |
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| Institution: | 1. Département de Biologie Moléculaire, Université de Genevé, 30, Quai Ernest-Ansermet, 1211- Genéve 4 Switzerland;2. Département de Pathologie, Université de Genéve, 30, Quai Ernest-Ansermet, 1211-Genéve 4 Switzerland |
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| Abstract: | Mouse and rabbit globin and immunoglobulin gene sequences, which had been synthesized in vitro from eukaryotic mRNAs and inserted into plasmids, have been examined in the electron microscope. The size of the inserted β rabbit and α and β mouse globin DNA sequences has been estimated as 620 base pairs while the size of the inserted α rabbit globin DNA sequences was found to be about 490 base pairs. Heteroduplex analysis has revealed no structural abnormalities at the insertion sites of the chimeric plasmids except in the case of a plasmid containing an immunoglobulin light chain gene sequence of about 830 bases, in which a 3 kb deletion adjacent to the insertion site was observed. |
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| Keywords: | Hybrid plasmids electron microscopy of heteroduplexes α and β globin DNA immunoglobulin light chain DNA kb 1000 base pairs of double-stranded DNA or 1000 bases of single-stranded DNA |
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