Thermal cycle dideoxy DNA sequencing |
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Authors: | Barton E Slatko |
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Institution: | (1) New England Biolabs, Inc., 32 Tozer Road, 01915 Beverly, MA |
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Abstract: | Thermal cycle dideoxy DNA sequencing eliminates the requirements for independent primer annealing and double-stranded DNA
denaturation steps. The method enables sequencing from nanogram amounts of DNA from double-stranded and single-stranded PCR
products, and plasmid or phage DNA templates. Thermal cycle sequencing also enables direct sequencing from bacterial colonies
or phage plaques. Protocols using the Vent exo− DNA polymerase, helpful suggestions, and a troubleshooting guide are also presented. |
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Keywords: | DNA Sequencing PCR Vent exo− polymerase thermal cycle sequencing |
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