Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc1 site of the mitochondrial respiratory chain |
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| Authors: | M Ksenzenko A A Konstantinov G B Khomutov A N Tikhonov E K Ruuge |
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| Institution: | 1. A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 117234, USSR;2. Faculty of Physics, Moscow State University, Moscow 117234, USSR |
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| Abstract: | Antimycin, 2-nonyl-4-hydroxyquinoline N-oxide and funiculosin induce O.2(-) generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3-dimercaptopropanol inhibit O.2(-) generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulates the reaction initiated by antimycin. The results indicate that auto-oxidation of unstable ubisemiquinone formed in centre o of the Q-cycle generates most of the O.2(-) radicals in the cytochrome bc1-site of the mitochondrial respiratory chain. |
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| Keywords: | Electron-transfer inhibitor Superoxide generation Respiratory chain of mitochondria Ubisemiquinone auto-oxidation NoHOQnO BAL British Anti-Lewisite (2 3-dimercaptopropanol) SMP sub-mitochondrial particles SOD superoxide dismutase TTFA α-thenoyltrifluoroacetone |
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