微卫星位点BEM06与BEM23鉴别烟粉虱B型与Q型的有效性

烟粉虱Bemisia tabaci (Gennadius) B型与Q型是烟粉虱复合种中入侵性较强、分布较广的2种生物型, 当前在许多地区混合发生。这2种生物型的快速鉴别对其种群动态调查及入侵生态学研究具有重要价值。为了验证微卫星位点BEM06与BEM23鉴别B型与Q型烟粉虱的有效性, 本研究分析了这2个微卫星位点的等位基因在国内外17个B型、4个Q型、3个非B/Q型烟粉虱种群的分布特点。结果表明: 这2个微卫星位点的联合使用可鉴别B型与Q型烟粉虱, 但是无法有效地将B型、Q型与一些非B/Q型烟粉虱某些个体区分开来。结果提示: 利用微卫星位点BEM06与BEM23鉴别B型与Q型烟粉虱具有一定的局限性, 尤其是田间烟粉虱存在其他生物型时需要慎重使用这种鉴别方法。

Validity of microsatellite loci BEM06 and BEM23 to differentiate Bemisia tabaci biotypes B and Q (Hemiptera: Aleyrodidae)

Bemisia tabaci (Gennadius) biotypes B and Q are the two most invasive and widely distributed biotypes within the B. tabaci complex and occur mixedly in many regions at present. The rapid differentiation of the two biotypes is significant to the research of their population dynamics and invasion ecology. In order to verify the validity of microsatellite loci BEM06 and BEM23 to differentiate B. tabaci biotypes B and Q, the alleles of the two loci in the 17 B-biotype populations, 4 Q-biotype populations and 3 non-B/Q biotype populations were analyzed. The results showed that although the two loci used in combination could differentiate B. tabaci biotypes B and Q, they could not differentiate B/Q-biotype individuals from some non-B/Q biotype individuals effectively. The results suggest that the method to differentiate the biotypes B and Q with the two microsatellite loci has its limitations, especially when used for field populations in which other biotypes exist.