Studies on the human chromosome 3 centromere with a newly cloned alphoid DNA probe |
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| Authors: | O Delattre A Bernard B Malfoy F Marlhens E Viegas-Pequignot C Brossard O Haguenauer N Creau-Goldberg V C N'guyen B Dutrillaux |
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| Affiliation: | Section de Biologie, Institut Curie, Paris, France. |
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| Abstract: | Starting from a chromosome-specific DNA library, we have isolated a human chromosome-specific satellite DNA sequence. This sequence of 635 base pairs (bp) consists of 3.7 alpha DNA monomers of 170-171 bp. Under high stringency it hybridizes to the centromere of chromosome 3 in a region composed of 2,750 bp tandem repeats characterized by the regular spacing of Hind III and TaqI restriction enzyme recognition sites. It has diverged and undergone amplification after the human speciation. The amplification allows an easy monitoring of the chromosome 3 centromere by in situ hybridization with a nonradioactive probe. |
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