Most MCF7 and SK-OV3 cells were deprived of their stem nature by Hoechst 33342 |
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| Authors: | Zhong Yong Zhou Chunxia Ma Wenbo Wang Dongmei Guo Sujuan Su Xiaosan Zhang Shuren |
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| Affiliation: | Department of Immunology, Cancer Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Pan Jia Yuan Nan Li, Chao Yang District, Beijing 100021, China |
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| Abstract: | Using clonal and population analysis, we found that the MCF7 and SK-OV3 were composed mainly of cancer stem cells. Together with SP analysis, we found that both SP and NSP cells of MCF7 and SK-OV3 contained different proportions of cancer stem cells. In addition, we discovered that MCF7 SP cells were able to generate non-SP cells, and conversely non-SP cells generated SP cells; Moreover, we showed that Hoechst 33342 and FACS were harmful to the clonogenicity of MCF7 and SK-OV3 cells. |
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| Keywords: | FACS fluorescence activated cell sorting |
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