| DNA拼接技术研究进展 |
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| 引用本文： | 张双华,孙源,张治洲. DNA拼接技术研究进展[J]. 生命科学, 2013, 0(11): 1135-1143 |
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| 作者姓名： | 张双华 孙源 张治洲 |
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| 作者单位： | 哈尔滨工业大学威海海洋科学与技术学院山东省海洋船舶防污工程技术研究中心,威海264209 |
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| 基金项目： | 国家自然科学基金项目（31071170）;GRED-BIO基金,HIT（hitwh200904）;985基金和HITNSRIF（2011101） |
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| 摘 要： | 基因组合成是合成生物学的一个重要环节，其发展将会对未来的生物、医药、农业、能源等方面的发展产生巨大的推动作用，同时DNA拼接作为基因组合成所需要的一种关键技术也日臻完备。回顾了多种DNA拼接技术，并对其中最具发展潜力的几种方法作了综述，探讨不同DNA拼接技术的原理和特点。
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| 关 键 词： | 基因组合成 DNA拼接 Gibson拼接 酵母同源重组 |
Research progress in DNA assembly technologies |
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| ZHANG Shuang-Hua,SUN Yuan,ZHANG Zhi-Zhou. Research progress in DNA assembly technologies[J]. Chinese Bulletin of Life Sciences, 2013, 0(11): 1135-1143 |
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| Authors: | ZHANG Shuang-Hua SUN Yuan ZHANG Zhi-Zhou |
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| Affiliation: | (School of Marine Science and Technology, Marine Antifouling Engineering Technology Center of Shangdong Province, Harbi Institute of Technology, Weihai 264209, China) |
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| Abstract: | Genome synthesis constitutes an important part of synthetic biology, and its development will bring huge impact on many disciplines such as biology, medicine, pagriculture, energy and so on. By now DNA assembly is a key technology for genome or long DNA synthesis. Some DNA assembly technologies have acquired strong functional progress. In this review, a series of DNA assembly techniques are described and discussed in the context of their principles, potentials and characteristics. |
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| Keywords: | genome synthesis DNA assembly Gibson assembly yeast homologous recombination |
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