Characteristics of phospholipase D in reverse micelles of Triton X-100 and phosphatidylcholine in diethyl ether |
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Authors: | S Subramani N Dittrich F Hirche R Ulbrich-Hofmann |
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Institution: | (1) Department of Biochemistry/Biotechnology, Martin-Luther University, D-06099 Halle, Federal Republic of Germany |
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Abstract: | Summary Phospholipase D, from cabbage, is active in reverse micelles formed from its substrate phosphatidylcholine and Triton X-100 in diethyl ether. The activity is optimum at w0=12.5. The increase of the molar ratio of Triton X-100/substrate from 1:4 to 2:1 results in an activity decrease by 25 %. At 136 mM Triton X-100 the KM value in reverse micelles is 136 mM, whereas it is 0.40 mM in the aqueous system containing SDS. |
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