| 白念珠菌破壁方法的研究应用进展 |
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| 引用本文： | 王晓飞,;张璐璐,;曹永兵,;姜远英. 白念珠菌破壁方法的研究应用进展[J]. 中国真菌学杂志, 2014, 0(5): 310-315 |
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| 作者姓名： | 王晓飞, 张璐璐, 曹永兵, 姜远英 |
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| 作者单位： | [1]福建中医药大学药学院中药学教研室,福州350108; [2]中国人民解放军第二军医大学药学院新药研究中心,上海200433 |
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| 基金项目： | 国家自然科学基金（81173635,81273556） |
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| 摘 要： | 细胞壁作为真菌中特殊和必须的细胞结构，相对于哺乳动物的细胞膜更加坚硬，难以用简单的方法使其充分破碎。因此，达到理想的破壁效果是白念珠菌研究中的关键步骤之一。在提取白念珠菌RNA、DNA和蛋白质等细胞组分的过程中，为获得足量和稳定的实验样品。该文对多种白念珠菌破壁方法作一综述，以便为白念珠菌相关研究提供适用、高效的破壁方案。
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| 关 键 词： | 白念珠菌 细胞壁 破碎 |
The research progress of Candida albicans wall-breaking method |
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| Affiliation: | WANG Xiao-fei, ZHANG Lu-lu, CAO Yong-bing, JIANG Yuan-ying (1. Department of Traditional Chinese materia medica,School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China ;2. R＆D Center of New Drug, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
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| Abstract: | Cell wall as a special and necessary cell structure of fungus itˊs hard to make it fully broken in a simple way There-fore,to achieve the ideal wall-breaking effect is one of the key steps in the study of Candida albicans In extraction such as RNA, DNA and protein components of Candida albicans ,in order to obtain sufficient and stable experimental samples,the researchers shoulduse different methods breaking the cell wall according to different research purposes This paper summarizes a variety of Candida albicans wall-breaking methods,so as to provide applicable and efficient solution of wall-breaking for related research in Candida albicans. |
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| Keywords: | Candida albicans cell wall breaking |
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