Schistosoma japonicum: localization of calpain in the penetration glands and secretions of cercariae |
| |
Authors: | Kumagai Takashi Maruyama Haruhiko Hato Mariko Ohmae Hiroshi Osada Yoshio Kanazawa Tamotsu Ohta Nobuo |
| |
Affiliation: | Department of Parasitology and Tropical Public Health, The University of Occupational and Environmental Health Japan, Kitakyusyu, Japan. tkuma@med.uoeh-u.ac.jp |
| |
Abstract: | A monoclonal antibody was generated against the large subunit of Schistosoma japonicum calpain to study the localization and possible function of the molecule in vivo. Mice were immunized with recombinant S. japonicum calpain and polyclonal antisera and a monoclonal antibody specific to schistosome calpain was obtained. In immunohistochemistry, a monoclonal antibody against S. japonicum calpain, KG-2E11, bound weakly to calpain expressed at the surface of adult worm tegument, however, it bound strongly to the cercarial secretions ("footprints") of S. japonicum, emitted from the penetration glands. The present study indicates that calpain is multifunctional as it is expressed at various locations in different developmental stages. Calpain-based vaccines could thus possibly induce protective immunity against cercariae and the following early developing stages. |
| |
Keywords: | IFN-γ, interferon-gamma CBB, Coomassie brilliant blue ELISA, enzyme-linked immunosorbent assay FBS, fetal bovine serum mAb, monoclonal antibody PBS, phosphate-buffered saline PVDF, polyvinylidene difluoride SEA, soluble egg antigen SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis SWAP, soluble adult worm antigen. |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|