Genetic transformation of willows (Salix spp.) byAgrobacterium tumefaciens |
| |
Authors: | Tiina Vahala Priska Stabel Tage Eriksson |
| |
Institution: | (1) Department of Physiological Botany, University of Uppsala, Box 540, S-75121 Uppsala, Sweden |
| |
Abstract: | Anin vitro transformation method has been developed for stem explants of fast-growing willow clones (Salix spp.) usingAgrobacterium tumefaciens as a vector. Transformants obtained with the strains C58 and GV3101 (pGV3851::pLD1) were selected on hormone-free medium and on medium containing kanamycin, respectively. Transformation was confirmed by Southern blot analysis and nopaline assay. Inoculation of green-house grown plants with nopaline and octopine wildtype strains and shoot or root inducing mutant strains caused undifferentiated tumors at a frequency of 0 to 80%, depending on theSalix genotype and the bacterial strain used.Abbreviations BA
benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- Km
kanamycin
- NPT
neomycin phosphotransferase |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|