| BEL-7404人肝癌细胞甲胎蛋白基因表达的原位杂交与免疫细胞化学研究 |
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| 引用本文： | 王兴旺,胥彬.BEL-7404人肝癌细胞甲胎蛋白基因表达的原位杂交与免疫细胞化学研究[J].中国组织化学与细胞化学杂志,1997(3). |
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| 作者姓名： | 王兴旺 胥彬 |
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| 作者单位： | 中国科学院上海药物研究所 |
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| 摘 要： | 本文用地高辛标记的甲胎蛋白（ＡＦＰ）ｃＤＮＡ探针原位杂交和免疫细胞化学方法观察到，几乎所有的ＢＥＬ－７４０４人肝癌细胞均能表达ＡＦＰｍＲＮＡ和相应的蛋白。
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| 关 键 词： | 原位杂交 免疫细胞化学 甲胎蛋白 基因表达 人肝癌细胞 |
IN SITU HYBRIDIZATION AND IMMUNOCYTOCHEMISTRY FOR THE INVESTIGATION OF ALPHA-FETOPROTEIN GENE EXPRESSION IN HUMAN BEL 7404 HEPATOMA CELLS |
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| Wang Xingwang,Xu Bin.IN SITU HYBRIDIZATION AND IMMUNOCYTOCHEMISTRY FOR THE INVESTIGATION OF ALPHA-FETOPROTEIN GENE EXPRESSION IN HUMAN BEL 7404 HEPATOMA CELLS[J].Chinese Journal of Histochemistry and Cytochemistry,1997(3). |
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| Authors: | Wang Xingwang Xu Bin |
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| Abstract: | In situ hybridization with digoxigenin-labelled alpha-fetoprotein (AFP) cDNA probe and immunocytochemistry were used to demonstrate AFP mRNA and AFP immunoreactivity in human BEL 7404 hepatoma cells. Almost all the hepatoma cells could express AFP mRNA and AFP protein. |
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| Keywords: | In situ hybridization Immunocytochemistry Alpha-fetoprotein Gene expression Human hepatoma cells |
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