Assessment of Plant Lectin Antifungal Potential Against Yeasts of Major Importance in Medical Mycology |
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Authors: | Gabriel Baracy Klafke Gustavo Marçal Schmitt Garcia Moreira Leonardo Garcia Monte Juliano Lacava Pereira Tchana Martinez Brandolt Melissa Orzechowski Xavier Tatiane Santi-Gadelha Odir Antonio Dellagostin Luciano da Silva Pinto |
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Institution: | 1. Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Campus Universitário Cap?o do Le?o, CP 354, Pelotas city, RS, CEP 96010-900, Brazil 2. Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Campus Saúde, CP 102, Rio Grande city, RS, CEP 96203-900, Brazil 3. Departamento de Biologia Molecular, Universidade Federal da Paraíba, Cidade Universitária, Jo?o Pessoa city, PB, CEP 58059-900, Brazil 4. Laboratório de Biotecnologia Vegetal e Prote?mica, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Cap?o do Le?o, RS, 96010-900, Brazil
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Abstract: | The search for new compounds with antifungal activity is accelerating due to rising yeast and fungal resistance to commonly prescribed drugs. Among the molecules being investigated, plant lectins can be highlighted. The present work shows the potential of six plant lectins which were tested in vitro against yeasts of medical importance, Candida albicans, Candida tropicalis, Candida parapsilosis, Cryptococcus gattii, Cryptococcus neoformans, Malassezia pachydermatis, Rhodotorula sp. and Trichosporon sp. Broth microdilution susceptibility testing was performed in accordance with standard protocols to evaluate antifungal activity. Minimum inhibitory concentration (MIC) was determined at 80 % yeast growth inhibition, whereas the minimum fungicidal concentration (MFC) was evaluated after making the subcultures of each dilution. Only C. parapsilosis growth was inhibited by the lectins tested. Abelmoschus esculentus lectin showed the highest MIC (0.97 μg ml?1). Lectins from Canavalia brasiliensis, Mucuna pruriens and Clitoria fairchildiana presented the highest MFC at (3.90 μg ml?1). These results encourage further studies with wider yeast strain selections, and open new perspectives for the development of pharmacological molecules. |
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