Occurrence and distribution of virulent strains of Vibrio parahaemolyticus in seafoods marketed from Cochin (India) |
| |
| Authors: | Rekha D Chakraborty P K Surendran |
| |
| Institution: | (1) Microbiology Fermentation and Biotechnology Division, Central Institute of Fisheries Technology, Matsyapuri, Cochin, India;(2) Present address: Division of Crustacean Fisheries, Central Marine Fisheries Research Institute, Ernakulam North P. O, P. B. no. 1603, Cochin, 682018, Kerala, India |
| |
| Abstract: | This study was aimed for the detection of Vibrio parahaemolyticus by biochemical and molecular methods in seafood samples collected from the markets of Cochin located at the southwest coast
of India. A total of seventy-two V. parahaemolyticus cultures were isolated by selecting sucrose and cellobiose non-fermenting colonies. All the biochemically confirmed strains
were found to have 368-bp toxR gene fragment, while an additional 24% of the samples were confirmed as V. parahaemolyticus by toxR based polymerase chain reaction (PCR) from enrichment broths. PCR based methods are used to detect tdh, trh, and orf8 genes for the identification of pathogenic and pandemic V. parahaemolyticus. Only one out of two urease positive isolates amplified the trh (500bp) gene. About 10% of the isolates showed weak haemolysis and none were found to amplify tdh (269 bp) and orf8 (746 bp) genes, thus indicating the meager incidence of pandemic strains from this area. The incidence of trh positive isolates from market samples signals towards the adoption of stringent seafood safety measures for the products
meant for human consumption. |
| |
| Keywords: | Vibrio parahaemolyticus Seafood Polymerase chain reaction toxR trh |
| 本文献已被 SpringerLink 等数据库收录！ |
|
