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Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme |
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| Authors: | K Akai K Yagi H F Tiano Y C Pan M Shimizu K Fong R A Jungmann S S Li |
| Affiliation: | 1. Laboratory of Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, U.S.A.;2. Department of Molecular Biology, Northwestern University Medical School, Chicago, IL 60611, U.S.A.;1. Universidad Nacional de Córdoba, Facultad de Ciencias Exactas Físicas y Naturales, Departamento de Construcciones Civiles, Córdoba, Argentina;2. CONICET - Universidad Nacional de Córdoba, Instituto de Estudios Avanzados en Ingeniería y Tecnología (IDIT), Córdoba, Argentina;3. Universidad Nacional de Córdoba, Facultad de Ciencias Exactas Físicas y Naturales, Departamento de Química Industrial y Aplicada, Córdoba, Argentina |
| Abstract: | A mouse lactate dehydrogenase-A cDNA was isolated and it was shown to contain the 393bp of the protein-coding sequence and 488bp of the 3' untranslated region. The amino acid sequence deduced from its open reading frame provided independent evidence for the sequence of residues 201-331 of mouse LDH-A subunit (muscle). This cDNA clone was used as a probe to isolate a mouse genomic clone containing a truncated, processed LDH-A pseudogene. This pseudogene showed 81.6% homology at 713 positions compared with the LDH-A cDNA sequence. The divergence of this pseudogene was estimated to have occurred 39 million years ago. |
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