Isolation of a new heterolobosean amoeba from a rice field soil: Vrihiamoeba italica gen. nov., sp. nov. |
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| Authors: | Jun Murase Michio Kawasaki Johan F De Jonckheere |
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| Institution: | 1. Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601 Nagoya, Japan;2. Research Unit for Tropical Diseases, de Duve Institute for Cellular Pathology, B1200 Brussels, Belgium;1. Department of Terrestrial Ecology, Institute of Zoology, University of Cologne, 50674 Cologne, Germany;2. College of Resources and Environmental Sciences, China Agricultural University, 100193 Beijing, China;3. de Duve Institute, 1200 Brussels, Belgium;4. Scientific Institute of Public Health, 1050 Brussels, Belgium;1. Department of Zoology and Ecology, Penza State University, Krasnaya str. 40, 440026 Penza, Russia;2. Laboratory of Soil Biology, Institute of Biology, University of Neuchâtel, rue Emile Argand 11, CH-2000 Neuchâtel, Switzerland;3. Jardin Botanique de Neuchâtel, Pertuis-du-Sault 56-58, CH-2000 Neuchâtel, Switzerland;4. Science Research Center, Hosei University, 2-17-1 Fujimi, Chiyoda-ku, 102-8160 Tokyo, Japan;5. Laboratory of Structural and Functional Organisation of Forest Ecosystems, Center for Problems of Ecology and Productivity of Forests, Russian Academy of Sciences, Profsoyuznaya str. 84/32, 117997 Moscow, Russia;1. Aix Marseille Université, Université de Toulon, CNRS, IRD, Mediterranean Institute of Oceanography (MIO), 13288 Marseille, France;2. Sorbonne Universités, UPMC, Université Paris 06, CNRS, Laboratoire d’Océanographie de Villefranche (LOV), 181 Chemin du Lazaret, 06230 Villefranche-sur-Mer, France;3. Marine Science Institute, University of California Santa Barbara, Santa Barbara, CA 93106, USA;4. Department of Marine Sciences, University of Georgia, Athens, GA 30602, USA |
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| Abstract: | A heterolobosean amoeba strain 6_5F was isolated from an Italian rice field soil. Although 18S rRNA gene sequence analysis demonstrated that the new isolate was closely related to Stachyamoeba sp. ATCC 50324, further molecular analysis and morphological observation showed distinct differences amongst the two. The 5.8S rRNA gene was successfully amplified and sequenced for strain 6_5F but not for strain ATCC 50324. Trophozoites of strain ATCC 50324 transform into flagellate forms in the late stage of incubation before encystment, while strain 6_5F do not show flagellate forms under different conditions of the flagellation test. Light and electron microscopic observation showed the structural difference of cysts of strain 6_5F from strain ATCC 50324 and also from the type strain Stachyamoeba lipophora. The results show that the strain 6_5F is distinct from Stachyamoeba spp. and we propose a new genus and species for this isolate, Vrihiamoeba italica gen. nov., sp. nov. |
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