XPC silencing in normal human keratinocytes triggers metabolic alterations through NOX-1 activation-mediated reactive oxygen species |
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Authors: | Rezvani Hamid Reza Rossignol Rodrigue Ali Nsrein Benard Giovanni Tang Xiuwei Yang Hee Seung Jouary Thomas de Verneuil Hubert Taïeb Alain Kim Arianna L Mazurier Frédéric |
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Institution: | Department of Dermatology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA. hamidreza.rezvani@u-bordeaux2.fr |
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Abstract: | Cancer cells utilize complex mechanisms to remodel their bioenergetic properties. We exploited the intrinsic genomic stability of xeroderma pigmentosum C (XPC) to understand the inter-relationships between genomic instability, reactive oxygen species (ROS) generation, and metabolic alterations during neoplastic transformation. We showed that knockdown of XPC (XPC(KD)) in normal human keratinocytes results in metabolism remodeling through NADPH oxidase-1 (NOX-1) activation, which in turn leads to increased ROS levels. While enforcing antioxidant defenses by overexpressing catalase, CuZnSOD, or MnSOD could not block the metabolism remodeling, impaired NOX-1 activation abrogates both alteration in ROS levels and modifications of energy metabolism. As NOX-1 activation is observed in human squamous cell carcinomas (SCCs), the blockade of NOX-1 could be a target for the prevention and the treatment of skin cancers. |
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