A chimeric peptide of intestinal trefoil factor containing cholesteryl ester transfer protein B cell epitope significantly inhibits atherosclerosis in rabbits after oral administration |
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Authors: | Qi Gaofu Li Jingjing Wang Shengying Xin Shanshan Du Peng Zhang Qingye Zhao Xiuyun |
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Affiliation: | a State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China b Hubei Province Key Lab of Bio-Technology of Traditional Chinese Medicine, Hubei University, Wuhan 430062, PR China |
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Abstract: | Vaccination against cholesteryl ester transfer protein (CETP) is proven to be effective for inhibiting atherosclerosis in animal models. In this study, the proteases-resistant intestinal trefoil factor (TFF3) was used as a molecular vehicle to construct chimeric TFF3 (cTFF3) containing CETP B cell epitope and tetanus toxin helper T cell epitope. It was found that cTFF3 still preserved a trefoil structure, and can resist proteases digestion in vitro. After oral immunization with cTFF3, the CETP-specific IgA and IgG could be found in intestine lavage fluid and serum, and the anti-CETP antibodies could inhibit partial CETP activity to increase high-density lipoprotein cholesterol, decrease low-density lipoprotein cholesterol, and inhibit atherosclerosis in animals. Therefore, TFF3 is a potential molecular vehicle for developing oral peptide vaccines. Our research highlights a novel strategy for developing oral peptide vaccines in the future. |
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Keywords: | Intestinal trefoil factor Oral peptide vaccine CETP Atherosclerosis |
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