| 酿酒酵母by1.1b产D-(-)-扁桃酸脱氢酶的发酵条件优化 |
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| 引用本文： | 严 芬,王 茜,林子琳,郭养浩. 酿酒酵母by1.1b产D-(-)-扁桃酸脱氢酶的发酵条件优化[J]. 微生物学通报, 2008, 35(1): 54-58 |
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| 作者姓名： | 严 芬 王 茜 林子琳 郭养浩 |
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| 作者单位： | 福州大学药物生物技术和工程研究所,福州,350002 |
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| 基金项目： | 福建省科技计划重点项目(No. 2003H023) |
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| 摘 要： | 对一株产D-(-)-扁桃酸对映选择性脱氢酶的酿酒酵母菌(Saccharomyces cerevisiae sp.strain by1.1b)发酵产酶条件进行了优化.研究各种碳源、氮源及无机盐对产酶的影响,应用正交试验优化发酵培养基组成,结果为:蛋白胨60 g/L,麦芽糖30 g/L,MgSO4 0.5 g/L,ZnSO4 0.01 g/L,KCl 1.0 g/L.优化后酶产量提高了7.9倍(由2.56 U/mL增至20.21 U/mL).摇瓶培养最佳条件为:装液量40%,发酵pH 6.5,接种量10%,发酵温度30℃.考察了细胞生长及产酶的时间进程,最佳培养时间为25 h.
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| 关 键 词： | D-(-)-扁桃酸 D-(-)-扁桃酸脱氢酶 酿酒酵母 产酶条件 酿酒酵母 扁桃酸脱氢酶 发酵温度 条件优化 Saccharomyces cerevisiae Optimization Strain Biosynthesis Efficient Culture Conditions 培养时间 时间进程 细胞生长 考察 接种量 装液量 最佳 摇瓶培养 产量提高 麦芽糖 |
| 修稿时间： | 2007-04-03 |
Optimization of Saccharomyces cerevisiae sp. Strain by1.1b Culture Conditions for Efficient Biosynthesis of D-(-)-mandelate Dehydrogenase |
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| YAN Fen,WANG Qian,LIN Zi-Lin and GUO Yang-Hao. Optimization of Saccharomyces cerevisiae sp. Strain by1.1b Culture Conditions for Efficient Biosynthesis of D-(-)-mandelate Dehydrogenase[J]. Microbiology China, 2008, 35(1): 54-58 |
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| Authors: | YAN Fen WANG Qian LIN Zi-Lin GUO Yang-Hao |
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| Affiliation: | Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou, 350002;Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou, 350002;Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou, 350002;Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou, 350002 |
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| Abstract: | The culture conditions of Saccharomyces cerevisiae sp.strain by 1.1 b were optimized for the production of D-(-)-mandelate dehydrogenase which is useful for the asymmetric bioreduction of benzoylformate to form D-(-)-mandelate.The optimum medium(per liter)consistes of 60 g peptone,30 g maltose, 0.5 g MgSO_4,0.01 g ZnSO_4,1.0 g KCl.After optimization of the culture medium,the enzyme production in shake flasks is enhanced from 2.56 to 20.21 U/L.The optimum fermentation conditions were determined as follows:me... |
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| Keywords: | D-(-)-mandelate D-(-)-mandelate dehydrogenase Saccharomyces cerevisiae sp. Fermentation condition |
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