The malate synthase gene of cucumber |
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| Authors: | Ian A. Graham Laura M. Smith John W. S. Brown Christopher J. Leaver Steven M. Smith |
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| Affiliation: | (1) Department of Botany, The Kings Buildings, University of Edinburgh, Mayfield Road, EH9 3JH Edinburgh, Scotland, UK;(2) Present address: Department of Biochemistry, University of Leeds, LS2 9JT Leeds, England, UK;(3) Present address: Department of Biological Sciences, The University, DD1 4HN Dundee, Scotland, UK |
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| Abstract: | The complete sequences of a full-length cDNA clone and a genomic clone encoding the Cucumis sativus glyoxysomal enzyme malate synthase, have been determined. The sequences have enabled us to identify putative control regions at the 5 end of the gene, three introns, and possible alternative polyadenylation sites at the 3 end. The deduced amino acid sequence predicts a polypeptide of 64961 molecular weight, which has 48% identity with that of Escherichia coli. Comparison of the sequence of malate synthase from cucumber with that from E. coli and with other glyoxysomal and peroxisomal enzymes, shows that a conserved C-terminal tripeptide is a common feature of those enzymes imported into microbodies. |
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| Keywords: | malate synthase gene structure glyoxylate cycle glyoxysomes Cucumis sativus |
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