| 西洋参悬浮细胞发酵工艺研究 |
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| 引用本文： | 张小兵,闫静辉,李亚璞,吴萌,李春生,陈英珠,程华. 西洋参悬浮细胞发酵工艺研究[J]. 生物技术通报, 2007, 0(5): 188-193 |
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| 作者姓名： | 张小兵 闫静辉 李亚璞 吴萌 李春生 陈英珠 程华 |
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| 作者单位： | 河北省生物研究所,石家庄,050081 |
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| 摘 要： | 探讨了西洋参悬浮细胞分步培养与稀土、D-半乳糖和甘露醇等诱导子对悬浮细胞生长及皂甙产量的影响。发现继代4d后换液一次再继续培养获得的培养物,在皂甙产率和糖利用率等方面优于连续培养;D-半乳糖作为诱导子,对悬浮培养的西洋参细胞生长、皂甙产率及皂甙的分泌等方面都有非常明显的促进作用。
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| 关 键 词： | 西洋参悬浮细胞 人参皂甙 分步培养 诱导子 |
| 修稿时间： | 2007-04-11 |
Studies on Fermentation of Suspension Cells of Panax quinquefolium |
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| Zhang Xiaobing,Yan Jinghui,Li Yapu,Wu Meng,Li Chunsheng,Chen Yingzhu,Cheng Hua. Studies on Fermentation of Suspension Cells of Panax quinquefolium[J]. Biotechnology Bulletin, 2007, 0(5): 188-193 |
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| Authors: | Zhang Xiaobing Yan Jinghui Li Yapu Wu Meng Li Chunsheng Chen Yingzhu Cheng Hua |
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| Affiliation: | Biology Institute of Hebei,Shijiazhuang 050081 |
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| Abstract: | The effects of a feeding culture and several elicitors on cell growth and saponin production in Panax quinquefolium suspension cell culture were investigated in the study.The results revealed that the feeding culture was better for the production of saponin than the continuous culture,and D-galactose as elicitor obviously stimulated the cell growth and saponin secretion. |
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| Keywords: | Suspension cell in Panax quinquefolium Ginseeng saponins Feeding culture Elicitor |
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