| 转基因大豆非同源对照模板QC-PCR检测方法的建立 |
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| 引用本文： | 徐景升,阙友雄,李峰,陈华墙,陈如凯. 转基因大豆非同源对照模板QC-PCR检测方法的建立[J]. 生物技术通报, 2007, 0(5): 156-159 |
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| 作者姓名： | 徐景升 阙友雄 李峰 陈华墙 陈如凯 |
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| 作者单位： | 1. 农业部甘蔗生理生态与遗传改良重点实验室,福建农林大学甘蔗研究所,福州,350002
2. 福建省立医院病理科室,福州,350002 |
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| 基金项目： | 福建省青年科技人才创新基金 |
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| 摘 要： | 建立了转基因大豆CP4EPSPS基因的非同源对照模板QC-PCR检测方法。非同源竞争对照构建方法如下:利用CP4EPSPS基因特异引物,以大肠杆菌基因组DNA为异源模板,在低严谨度PCR扩增,回收适宜DNA片段并克隆到pMD-8载体上。该片段与CP4EPSPS基因相应序列除两端引物序列完全相同外,其他部分没有同源性。
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| 关 键 词： | 竞争定量PCR 非同源对照 转基因检测 |
| 修稿时间： | 2007-08-08 |
A Method for the Detection of Transgenic Soybean Using QC-PCR with Non-Homologous Competitor |
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| Xu Jingsheng,Que Youxiong,Li Feng,Chen Huaqiang,Chen Rukai. A Method for the Detection of Transgenic Soybean Using QC-PCR with Non-Homologous Competitor[J]. Biotechnology Bulletin, 2007, 0(5): 156-159 |
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| Authors: | Xu Jingsheng Que Youxiong Li Feng Chen Huaqiang Chen Rukai |
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| Abstract: | In this paper,the non-homologous competitor QC-PCR system for the detection of transgenic soybean was established. The non-homologous competitor was constructed as following: the E.coli genomic DNA was low stringently amplified by cross-species PCR using CP4EPSPS-specific primers. The selected DNA fragment was reclaimed and cloned into pMD-8 T vector. The sequence of the competitor showed no homologous with the corresponding sequence of CP4EPSPS gene other than the primers at its ends. |
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| Keywords: | CP4EPSPS |
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