| 复合诱变法选育香兰素高转化率菌株 |
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| 引用本文： | 王昌禄,包全帅,周庆礼,陈勉华.复合诱变法选育香兰素高转化率菌株[J].生物技术通报,2007,0(5):184-187. |
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| 作者姓名： | 王昌禄 包全帅 周庆礼 陈勉华 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院,天津市食品加工工程中心,天津,300457 |
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| 摘 要： | 通过紫外线照射和He-Ne激光修复对香兰素生产菌链霉菌Streptomyces sp.L1936进行复合诱变,得到香兰素高产菌株Streptomyces sp.L1936-8。结果表明:出发菌株经过诱变处理后,脱乙酰酶酶活提高了75%,香兰素氧化酶酶活降低了37.5%,香兰素产量提高了33%。
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| 关 键 词： | 香兰素 链霉菌 紫外线-激光复合诱变 |
| 修稿时间： | 2007-05-08 |
Breeding of High Vanillin Transformation Ratio by Compound Mutagenesis |
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| Wang Changlu,Bao Quanshuai,Zhou Qingli,Chen Mianhua.Breeding of High Vanillin Transformation Ratio by Compound Mutagenesis[J].Biotechnology Bulletin,2007,0(5):184-187. |
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| Authors: | Wang Changlu Bao Quanshuai Zhou Qingli Chen Mianhua |
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| Abstract: | A high vanillin `yielding mutant Streptomyces sp.L1936-8 was obtained from its parent strain Streptomyces sp.L1936 by UV and He-Ne Laser mutagenesis.The activity of deacetylase was raised 75%.and the activity of vanillin oxidase was desecended 37.5%.The vanillin productivity of the mutant reached 5.0g/L,which was 33% higher than that of the parent strain. |
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| Keywords: | Vanillin Streptomyces UV-laser compound mutagenesis |
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