An extraction resistant tensile protein in the protoplasmic matrix of Physarum |
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| Authors: | D Gassner Z Shraideh K E Wohlfarth-Bottermann |
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| Affiliation: | 1. State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China;2. Department of Food Science, College of Food Science and Engineering, Jilin University, Changchun, China;3. Department of Respiratory Medicine, the First Hospital of Jilin University, Changchun, China;4. The Second Bethune Clinical Medical College of Jilin University, Changchun 130012, Jilin, People''s Republic of China;5. Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, P.R.China;6. Key Laboratory of Livestock and Poultry Multi-omics of MARA, P.R.China;1. Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China;2. Department of Neurosurgery & Laboratory of Neurosurgery West China Hospital Sichuan University, Chengdu 610041, PR China;1. Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, China;2. Cancer Precision Diagnosis and Treatment and Translational Medicine Hubei Engineering Research Center, Zhongnan Hospital of Wuhan University, Wuhan 430071, China;1. Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan, 430072, PR China;2. Institute for Advanced Studies, Wuhan University, Wuhan, 430072, PR China;3. Wuhan Research Centre for Infectious Diseases and Cancer, Chinese Academy of Medical Sciences, Wuhan, 430071, PR China |
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| Abstract: | A thorough extraction of plasmodia of Physarum polycephalum by sequential treatment with 1% Triton x-100, 0.6 M KI, 4% SDS plus 7 M urea leaves behind an elastic cell ghost, which represents a cytoplasmic matrix protein arranged as a continuous network in all cell regions. The protein is present in the ectoplasm as well as in the endoplasm. The extraction-resistant ghosts reveal filaments 2-3 nm in diameter, perform a conspicuous volume condensation upon the addition of mM-concentrations of di- and trivalent cations and can be partially solubilized in 4.5 M guanidinium chloride plus 25% 2-mercaptoethanol at 70 degrees C. SDS-gel electrophoresis shows a distinct band at 43,000 daltons and a faint high molecular weight component suggesting a similarity to muscle connection. |
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